The effectiveness of 1% cysteamine, 3% tranexamic acid, 2% galactomyces ferment filtrate and 4% niacinamide application using double layering technique as a brightening agent: Chromameter and Janus-III analysis


  • Anis Anwar Department of Dermatology and Venereology, Faculty of Medicine, Hasanuddin University
  • Siswanto Wahab
  • Asvina Anis Anwar
  • Natalia Widjaja
  • Tania Azhari
  • Thomas Utomo
  • Wong Lip Wih
  • Jonathan Kurnia Wijaya


Cysteamine, Double layering, Skin brightness, Topical skin lightening agent


Introduction: The combination of several topical agents with different vehicles or routes of administration is one of the therapeutic strategies that can be used to increase the effectiveness and possibly to reduce unwanted side-effects.   Objective: To assess the efficacy of 1% cysteamine, 3% tranexamic acid, 2% galactomyces ferment filtrate, and 4% niacinamide as a facial brightening agent using the double layering technique of serum and cream.   Methods: The open-label pre-post-test clinical trial was conducted for eight weeks using on 70 type-IV Fitzpatrick skin female participants between the ages of 31-50 years-old (mean age 37.8 years). Subjects were evaluated on a biweekly basis using chromameter and Janus-III Facial Analyzer.   Results: At the end of the study there were significant L* value increase from 52.10 at W0 to 56.40 at W4 (p=0.00) along with improvement on pigmented areas from 45.52 to 43.90 after eight weeks of treatment (p=0.00 and 0.01 respectively).   Conclusion: Application of 1% cysteamine, 3% tranexamic acid, 2% galactomyces ferment filtrate and 4% niacinamide using double layering technique is a promising combination for facial brightness with notable reduced side-effects.  


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How to Cite

Anwar A, Wahab S, Anwar AA, Widjaja N, Azhari T, Utomo T, Wih WL, Wijaya JK. The effectiveness of 1% cysteamine, 3% tranexamic acid, 2% galactomyces ferment filtrate and 4% niacinamide application using double layering technique as a brightening agent: Chromameter and Janus-III analysis. J Pak Assoc Dermatol [Internet]. 2024May6 [cited 2024May22];34(1):92-7. Available from:



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