Effects of L-Carnitine supplementation on relative gene expression of OCTN1, OCTN2, OCTN3 in mitochondria and skin in meldonium induced carnitine depleted male albino Wistar Rats
Keywords:Organic cation transporter OCTN protein, Rat, Gene expression, impaired glucose metabolism
AbstractIntroduction L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cells. However, further research is needed to fully understand the molecular mechanisms underlying these effects and their implications for human health. Methodology A total number of n=48 Albino Wistar rats were recruited and divided into four groups 12rats in each group. Group A was kept control whereas animals in remaining three groups were administered meldonium mixed with food in the form of powder. Meldonium was administered at 100mg/kg of body weight for the purpose of L-carnitine depletion for 10 days. Results Relative OCTN 1 gene expression in group A was noted as 1.2 ± 0.01 in contrast to the experimental groups B 2.1 ± 0.1, C 4.6 ± 0.7, D 6.2 ± 0.8 and E as 8.0 ± 0.9. Low and high doses of L-carnitine upregulated the expression of OCTN2 gene (P= 0.0001) Conclusion Overall, findings suggest that L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cells
Shahouzehi B, Masoumi-Ardakani Y, Fallah H, Aminizadeh S. Concomitant Administration of L-carnitine and Performing High-Intensity Interval Training Effects on the Genes Involved in Mitochondrial Fusion and Apoptosis in Rat Liver. Jordan Journal of Biological Sciences. 2023 Mar;16(1).
Koepsell H. Organic cation transporters in health and disease. Pharmacological reviews. 2020 Jan 1;72(1):253-319.
Koleske ML, McInnes G, Brown JE, Thomas N, Hutchinson K, Chin MY, Koehl A, Arkin MR, Schlessinger A, Gallagher RC, Song YS. Functional genomics of OCTN2 variants informs protein-specific variant effect predictor for Carnitine Transporter Deficiency. 2022 Nov 15;119(46):e2210247119.
Amiri R, Tabandeh MR, Hosseini SA. Novel cardioprotective effect of L-carnitine on obese diabetic mice: Regulation of chemerin and CMKLRI expression in heart and adipose tissues. Arquivos Brasileiros de Cardiologia. 2021 Oct 25;117:715-25.
Jing Z, Okubo H, Morishige JI, Xu P, Hasan N, Nagata N, Ando H. Lenvatinib causes reduced expression of carnitine/organic cation transporter 2 and carnitine deficiency in the skeletal muscle of rats. Toxicology Letters. 2022 Aug 1;366:17-25.
Li J, Liu L, Weng J, Yin TL, Yang J, Feng HL. Biological roles of l‐carnitine in oocyte and early embryo development. Molec
ular Reproduction and Development. 2021 Oct;88(10):673-85.
Gyawali A, Hyeon SJ, Ryu H, Kang YS. The alteration of l-carnitine transport and pretreatment effect under glutamate cytotoxicity on motor neuron-like NSC-34 lines. Pharmaceutics. 2021 Apr 14;13(4):551.
Dudicova S, Kacerovsky M, Ceckova M. Effect of inflammatory environment on the gene expression of OCTN1 (SLC22A4) and OCTN2 (SLC22A5) transporters in human trophoblast cell lines BeWo, HTR-8/SVneo and placental villous explants.
Jennaro TS, Puskarich MA, McCann MR, Gillies CE, Pai MP, Karnovsky A, Evans CR, Jones AE, Stringer KA. Using l‐Carnitine as a Pharmacologic Probe of the Interpatient and Metabolic Variability of Sepsis. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy. 2020 Sep;40(9):913-23.
Alhasaniah AH. l-carnitine: Nutrition, pathology, and health benefits. Saudi Journal of Biological Sciences. 2022 Dec 30:103555.
Pavelka K, Bruyere O, Cooper C, Kanis JA, Leeb BF, Maheu E, Martel-Pelletier J, Monfort J, Pelletier JP, Rizzoli R, Reginster JY. Diacerein: benefits, risks and place in the management of osteoarthritis. An opinion-based report from the ESCEO. Drugs & aging. 2016 Feb;33:75-85.
Lamhonwah AM, Tein I. Expression of the organic cation/carnitine transporter family (Octn1,-2 and-3) in mdx muscle and heart: Implications for early carnitine therapy in Duchenne muscular dystrophy to improve cellular carnitine homeostasis. Clinica Chimica Acta. 2020 Jun 1;505:92-7.
Kwon KC, Won JG, Myoung J, Kim MS, Cho Y, Park SW, Song YS. A Study on Evaluation of Exfoliation Efficacy and Erythema Relief by Carnitine Salicylate Ion Pair System. Korean Journal of Cosmetic Science. 2021;3(1):1-2.