Allogeneic mesenchymal stem cells therapy for burn wound rat model: A systematic review and meta-analysis
Keywords:allogeneic, mesenchymal, stem cells, burn, rat
AbstractBackground: Burn wounds are a burdensome problem affecting many people and cause high morbidity and mortality. Therefore, a more effective burn wound treatment is urgently needed. Objective: To evaluate the allogeneic mesenchymal stem cells’ efficacy in treating burn wounds in rats. Method: English articles were collected from the following databases: ProQuest, PubMed, EBSCOhost, SAGE, JSTOR, GARUDA, and Open Gray (January 1st, 2011, to March 29th, 2020). The inclusion criteria are original articles, rat burns experimental models, stem cells therapy using MSCs for experimental groups, non-functional solution, excipient or no treatment for control group, and healing rate of the burn wounds as the main outcome. Results and discussion: We found 9 articles in English. Hot plates or radiation were used to create burn model in 170 rats. The burn wounds degree ranged from partial (n = 2) to full-thickness (n = 4). Around 0.1–3.2 million stem cells were administered through various routes. The wound re-epithelialization rate was analyzed statistically and is shown more significant in the treatment group. Conclusion: Allogeneic MSCs may have benefits for treating burn wounds, although more experiments are still needed. RCTs are recommended to evaluate the efficacy and adverse effects of the MSCs treatment. Keywords: allogeneic; mesenchymal; stem cells; burn; rat
Keshavarzi A, Kardeh S, Dehghankhalili M, Varahram MH, Omidi M, Zardosht M, et al. Mortality and Body Mass Index in Burn Patients: Experience from a Tertiary Referral Burn Center in Southern Iran. World J Plast Surg. 2019;8(3):382–7.
Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Burn injury. Nat Rev Dis Prim. 2020;6(1).
Mozingo DW. Morbidity and Survival Probability in Burn Patients in Modern Burn Care. Yearb Surg. 2016;2016(4):63–5.
World Health Organization. The Global Burden of Disease: 2004 Update. World Health Organization, Geneva 2008.
World Health Organization. Burns – Fact Sheet. World Health Organization, Geneva 2018.
Esechie A, Bhardwaj A, Masel T, Raji M. Neurocognitive sequela of burn injury in the elderly. J Clin Neurosci. 2019;59:1–5.
Jeschke MG, Patsouris D, Stanojcic M, Abdullahi A, Rehou S, Pinto R, et al. Pathophysiologic Response to Burns in the Elderly. EBioMedicine. 2015;2(10):1536–48.
Pereira C, Gold W, Herndon D. Review paper: Burn coverage technologies: Current concepts and future directions. J Biomater Appl. 2007;22(2):101–21.
Quispe-Tintaya W. HHS Public Access. Physiol Behav. 2017;176(3):139–48.
Mahmood R, Mehmood A, Choudhery MS, Awan SJ, Khan SN, Riazuddin S. Human neonatal stem cell-derived skin substitute improves healing of severe burn wounds in a rat model. Cell Biol Int. 2019 Feb;43(2):147–57.
Oryan A, Alemzadeh E, Moshiri A. Burn wound healing: Present concepts, treatment strategies and future directions. J Wound Care. 2017;26(1):5–19.
Strauer BE, Kornowski R. Stem cell therapy in perspective. Circulation. 2003;107(7):929–34.
James R, Haridas N, Deb KD. Clinical applications of mesenchymal stem cells. Biointegration Med Implant Mater. 2019;101–16.
Singer DD, Singer AJ, Gordon C, Brink P. The effects of rat mesenchymal stem cells on injury progression in a rat model. Acad Emerg Med Off J Soc Acad Emerg Med. 2013 Apr;20(4):398–402.
Chen Y-W, Scutaru TT, Ghetu N, Carasevici E, Lupascu CD, Ferariu D, et al. The Effects of Adipose-Derived Stem Cell-Differentiated Adipocytes on Skin Burn Wound Healing in Rats. J Burn care Res Off Publ Am Burn Assoc. 2017;38(1):1–10.
Han Y, Sun T, Han Y, Lin L, Liu C, Liu J, et al. Human umbilical cord mesenchymal stem cells implantation accelerates cutaneous wound healing in diabetic rats via the Wnt signaling pathway. Eur J Med Res. 2019 Feb;24(1):10.
Zhang B, Wu X, Zhang X, Sun Y, Yan Y, Shi H, et al. Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/β-catenin pathway. Stem Cells Transl Med. 2015 May;4(5):513–22.
Jiang W, Zhou X-Y, Wang L, Liu Q, Liu C, Wang Y, et al. Skin-specifically transgenic expression of biologically active human cytoxic T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) in mice using lentiviral vector. Transgenic Res. 2012 Jun;21(3):579–91.
Iannaccone PM, Jacob HJ. Rats! DMM Dis Model Mech. 2009;2(5–6):206–10.
Mahmoud YI, Farag S. Kiwifruit ameliorates gentamicin induced histological and histochemical alterations in the kidney of albino mice. Biotech Histochem. 2017;92(5):357–62.
Hooijmans CR, Rovers MM, De Vries RBM, Leenaars M, Ritskes-Hoitinga M, Langendam MW. SYRCLE’s risk of bias tool for animal studies. BMC Med Res Methodol. 2014;14(1):1–9.
Cukjati D, Reberšek S, Miklavčič. A reliable method of determining wound healing rate. Med Biol Eng Comput. 2001;39(2):263–71.
Feng C-J, Lin C-H, Tsai C-H, Yang I-C, Ma H. Adipose-derived stem cells-induced burn wound healing and regeneration of skin appendages in a novel skin island rat model. J Chin Med Assoc. 2019 Aug;82(8):635–42.
Zhou X, Ning K, Ling B, Chen X, Cheng H, Lu B, et al. Multiple Injections of Autologous Adipose-Derived Stem Cells Accelerate the Burn Wound Healing Process and Promote Blood Vessel Regeneration in a Rat Model. Stem Cells Dev. 2019 Nov;28(21):1463–72.
Singer DD, Singer AJ, Gordon C, Brink P. The effects of rat mesenchymal stem cells on injury progression in a rat model. Acad Emerg Med. 2013;20(4):398–402.
Guo X, Xia B, Lu X-B, Zhang Z-J, Li Z, Li W-L, et al. Grafting of mesenchymal stem cell-seeded small intestinal submucosa to repair the deep partial-thickness burns. Connect Tissue Res. 2016 Sep;57(5):388–97.
Franck CL, Senegaglia AC, Leite LMB, De Moura SAB, Francisco NF, Ribas Filho JM. Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process. Stem Cells Int. 2019;2019.
Temnov A, Astrelina T, Rogov K, Moroz B, Lebedev V, Nasonova T, et al. Use of paracrine factors from stem cells to treat local radiation burns in rats. Stem Cells Cloning Adv Appl. 2018;11:69–76.
Chen YW, Scutaru TT, Ghetu N, Carasevici E, Lupascu CD, Ferariu D, et al. The Effects of Adipose-Derived Stem Cell-Differentiated Adipocytes on Skin Burn Wound Healing in Rats. J Burn Care Res. 2017;38(1):1–10.
Kakabadze Z, Chakhnashvili D, Gogilashvili K, Ediberidze K, Chakhnashvili K, Kalandarishvili K, et al. Bone Marrow Stem Cell and Decellularized Human Amniotic Membrane for the Treatment of Nonhealing Wound After Radiation Therapy. Exp Clin Transplant Off J Middle East Soc Organ Transplant. 2019 Jan;17(Suppl 1):92–8.
Imam RA, Rizk AAE. Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: Possible in vivo transdifferentiation into keratinocytes. Folia Morphol. 2019;78(4):798–808.
Nielsen CB, Duethman NC, Howard JM, Moncure M, Wood JG. Burns: Pathophysiology of Systemic Complications and Current Management. J Burn Care Res. 2017;38(1):e469–81.
Tiwari VK. Burn wound: How it differs from other wounds. Indian J Plast Surg. 2012;45(2):364–73.
Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8(2).
Oh EJ, Lee HW, Kalimuthu S, Kim TJ, Kim HM, Baek SH, et al. In vivo migration of mesenchymal stem cells to burn injury sites and their therapeutic effects in a living mouse model. J Control Release. 2018;279:79–88.
Sivamani RK, Garcia MS, Isseroff RR. Department of Dermatology, University of California, Davis, School of Medicine, Davis, California, 95616, USA, 2 Dermatology Service, Department of Veterans Affairs, Northern California Health Care System, Mather, CA 95655, USA. Front Biosci. 2007;12(1):2849–68.
Guillamat-Prats R. The Role of MSC in Wound Healing, Scarring and Regeneration. Cells. 2021;10(7):1729.
Ghieh F, Jurjus R, Ibrahim A, Geagea AG, Daouk H, El Baba B, et al. The Use of Stem Cells in Burn Wound Healing: A Review. Biomed Res Int. 2015;2015.
Henriksen JL, Sørensen NB, Fink T, Zachar V, Porsborg SR. Systematic Review of Stem-Cell-Based Therapy of Burn Wounds: Lessons Learned from Animal and Clinical Studies. Cells. 2020;9(12).
Babakhani A, Nobakht M, Pazoki Torodi H, Dahmardeh M, Hashemi P, Mohajer Ansari J, et al. Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength. Iran Biomed J. 2020 Mar;24(2):99–109.
Clover AJP, Kumar AHS, Isakson M, Whelan D, Stocca A, Gleeson BM, et al. Allogeneic mesenchymal stem cells, but not culture modified monocytes, improve burn wound healing. Burns. 2015;41(3):548–57.
Yi-Zhou Huang, Min Gou, Lin-Cui Da, Wen-Qian Zhang, and Hui-Qi Xie.Tissue Engineering Part B: Reviews.Dec 2020.555-570.