The outcome of intralesional vitamin D3 (alphacalcidol) injection in treatment of cutaneous warts


  • Asma Masood Department of Dermatology, Mayo Hospital, Lahore
  • Noshi Ilyas Department of Dermatology, Mayo Hospital, Lahore
  • Saira Riaz Department of Dermatology, Mayo Hospital, Lahore
  • Nehel Raza Department of Dermatology, Mayo Hospital, Lahore
  • Burhan Ashraf Department of Dermatology, Mayo Hospital, Lahore
  • Sara Mubeen Department of Dermatology, Mayo Hospital, Lahore
  • Muhammad Khalil-ur-Rehman Punjab University College of Pharmacy, University of the Punjab, Lahore
  • Shahbaz Aman Department of Dermatology, Mayo Hospital, Lahore
  • Tahir Jamil Department of Dermatology, Mayo Hospital, Lahore


Intralesional, Vitamin D3, Human papillomavirus (HPV), Cutaneous warts


Objective The objective of this study was to see the efficacy of intralesional vitamin D3 injection in treatment of warts. The efficacy was assessed in the term of ≥50% improvement in warts after treatment at 3rd month.   Methods This was a descriptive case series study and was completed in 6 months duration in dermatology department, Mayo hospital Lahore. A total of 97 cases of cutaneous warts fulfilling inclusion criteria were enrolled after getting approval from hospital ethical committee. All cases were included through OPD of Dermatology, Mayo Hospital Lahore after taking informed consent. Initially, lesions were anaesthetized with 0.1 ml of prilocaine (20 mg/ml). A 21-gauge syringe was used to inject 0.2 ml of vitamin D3 (7.5 mg/ml) slowly into the base of each wart. According to the treatment protocol, injections of vitamin D3 were given monthly with a maximum of 2 sessions. Per single session, the total amount injected into a patient was 7.5 mg. The whole procedure was done by consultant dermatologist having more than 5 years of experience. The treatment response ≥ 50% was recorded as per operational definition at 3rd month. All data was recorded by researchers themselves on the prescribed proforma.   Results The mean age of all cases was 40.94±11.53 with minimum and maximum age as 18 and 60 years. There were 56 (57.7%) male and 41 (42.3%) female cases. A total of 79 (81.4%) cases achieved efficacy of treatment while in 18 (18.6%) cases efficacy was not seen. Efficacy was seen in 77.5% cases who had duration of disease < 6 months and 92.3% of cases who had duration of disease since ≥ 6 months. The frequency of efficacy was statistically same in both genders and both duration of disease groups. These results showed that intralesional vitamin D3 was effective in the treatment of different types of cutaneous warts with high significance level.   Conclusion It was concluded that intralesional vitamin D3 injection was highly effective in treatment of cutaneous warts. Hence, intralesional vitamin D3 being an effective, inexpensive and safe treatment must be opted for this common problem in our society.  


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How to Cite

Masood A, Ilyas N, Riaz S, Raza N, Ashraf B, Mubeen S, Khalil-ur-Rehman M, Aman S, Jamil T. The outcome of intralesional vitamin D3 (alphacalcidol) injection in treatment of cutaneous warts. J Pak Assoc Dermatol [Internet]. 2023Oct.12 [cited 2023Nov.28];33(4):1280-4. Available from:



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