Expression of WNT1 signalling proteins after phototherapy exposure in vitiligo

Authors

  • Dian Ardiana Dermatology and Venereology Department, Faculty of Medicine University of Hang Tuah -Dr. Ramelan Naval Hospital
  • Nanny Herwanto Dermatology and Venereology Department, Faculty of Medicine Universitas Airlangga – Dr. Soetomo Teaching Hospital
  • Cita Rosita Sigit Prakoeswa Dermatology and Venereology Department, Faculty of Medicine Universitas Airlangga – Dr. Soetomo Teaching Hospital
  • Indropo Agusni Dermatology and Venereology Department, Faculty of Medicine Universitas Airlangga – Dr. Soetomo Teaching Hospital

Keywords:

Vitiligo, NB-UVB, melanocyte stem cells, Wnt1/ β-catenin, perifollicular repigmentation

Abstract

Background  Melanocyte stem cells (MelSCs) have been identified in the lower permanent portion of hair follicle and associated with perifollicular repigmentation pattern of vitiligo after Narrow Band-Ultraviolet B (NB-UVB) therapy. Wnt1 and β-catenin have been associated with UVB induced pigmentation in mice and hyperpigmented lesion commonly seen on sun-exposed areas. The increase of MelSCs in these two conditions suggested the role of Wnt1 in MelSCs differentiation, which increases the number of melanocytes. Purpose  To know the expression of hair follicle Wnt1 and β-catenin in NB-UVB induced vitiligo perifollicular repigmentation. Methods This clinical pre-experimental study used one group pre test-post test design. The dose of 390 mJ/cm2 DermaPalTM Daavlin NB-UVB was given twice a week for 2 months to 18 vitiligo patients. Biopsy was undertaken in vitiligo areas involving hair follicle before and after therapy and was assessed by immunohistochemistry technique. Results There was a significant difference (p<0.05) of vitiligo area size, the number of cells expressing Wnt1 and the number of MelSCs expressing β-catenin in hair follicle before and after NB-UVB irradiation. Conclusion There was an increment of hair follicle Wnt1 and β-catenin expression in vitiligo perifollicular areas before and after NB-UVB therapy. The finding of this study could be used for further research in perifollicular repigmentation mechanism. 

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Published

2019-10-21

How to Cite

1.
Ardiana D, Herwanto N, Prakoeswa CRS, Agusni I. Expression of WNT1 signalling proteins after phototherapy exposure in vitiligo. J Pak Assoc Dermatol [Internet]. 2019Oct.21 [cited 2026Jan.31];29(3):302-8. Available from: https://jpad.com.pk/index.php/jpad/article/view/1336

Issue

Vol. 29 No. 3 (2019): July - September

Section

Original Articles

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