Study of direct immunofluorescence in various autoimmune vesiculobullous disorders: an observational study

Megha Sharma, Krishnendra Varma


Objectives The aim of the study was to see the clinical, histological and immunological correlation of various immunobullous diseases and to evaluate the sensitivity of direct immunofluorescence (DIF) in specific diagnosis of various immunobullous diseases.


Methods 29 patients of immunobullous diseases attending skin OPD and IPD of a tertiary care hospital in Ujjain were included in the study and were thoroughly examined.


Results Out of 29, 26 cases showed DIF patterns concordant with histologic diagnosis. The sensitivity of DIF was 92.3% (12/13) in the pemphigus group, 75% (3/4) in bullous pemphigoid, 66.7% (2/3) in dermatitis herpetiformis, while it was 100% (3/3) each in linear IgA disease and chronic bullous dermatosis of childhood.


Conclusion DIF is essential for diagnosing autoimmune blistering diseases with the clinical and the histopathological overlap.



Direct immunofluorescence, immunobullous diseases, histopathology, sensitivity, diagnosis

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