The Platelet Rich Plasma (PRP) Preparation and Standardization of time interval for scalp hair loss

Abstract

Objectives of the Study: To evaluate PRP preparation and standardization of the technique and time interval between each PRP sessions particularly for scalp hair restoration. Study Design:              Cross sectional observational study conducted Place of Study:            Faisal Hospital, Peoples Colony, Faisalabad, Pakistan. Period of Study: One year and four months. From March 2019 to July 2020. Material and Methods: PRP Preparation Technique: Blood samples of 20 ml were taken utilizing EDTA tubes from the patients on whom the procedure was planned. Centrifugation 3000 Rev 1-3 minutes. PRP was prepared with plasma 2 ml (0.5 ml from each 4 tubes), activator 0.5 ml and lignocaine 0.5 ml; total 3 ml. The following injection technique was used for scalp: injection was administered in the subcutaneous tissua (below skin and connective tissue and above the galea aponeurosis, loose areolar tissue, pericranium).  With one centimetre distance approximately 15-20 injections were administered.  Interval between four sessions every two weeks, booster was given every three months. For medical treatment Fenestride and Minoxidil were given. The data was collected on a predesigned performa and analyzed using SPSS and Excel software applications. Results: In this study, total 147 consecutive PRP sessions were performed on 46 patients. The age range was from 19 years to 61 years. There were 37 male and 9 female patients in this study. With this technique of PRP preparation and injection technique and evidence based standardized set protocol for PRP time interval between each session, scalp hair stop falling, makes the hair stronger, regrowth of hair satisfactorily. The results showed satisfaction level up to 90 percent Conclusion: This technique is an easy way of PRP preparation. The result was with our standardized technique and time interval between each PRP sessions. Therefore, we recommend our method of PRP preparation technique and time interval between each PRP session.   Key Words: PRP, preparation, technique, standardization