Relationship of amino acid concentrations in blood with occurrence of dermatophytosis

Farzan Hayeri Khiavi, Aliasghar Gharachorlu

Abstract


Objective To compare the free amino acid concentrations of the blood in subjects suspected to tinea corporis with healthy individual.

 

Methods Blood samples from 40 adult (18-40 year) patients of mycologically-proven tinea corporis and 44 age- and sex-matched healthy controls were taken and assayed using high performance liquid chromatography for free amino acid levels.

 

Results 90% of patients showed higher free amino acids levels than controls. Methionine did not show significant difference in concentration between infected and healthy individuals. However, concentrations of glutamine, asparagine, phenylalanine, and tyrosine were significantly high in infected individuals with dermatophytes (p<0.005).

 

Conclusion Our finding provide information about the possible role of free amino acids in pathogenicity of dermatophytes.


Keywords


Dermatophytes, high performance liquid chromatography, tinea corporis

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References


Mendez-Tovar LJ. Pathogenesis of dermatophytosis and tinea versicolor. Clinics in dermatology. 2010;28(2):185-9.

Georg LK. Epidemiology of the dermatophytoses sources of infection, modes of transmission and epidemicity. Annals of the New York Academy of Sciences. 1960;89(1):69-77.

Rezaei-Matehkolaei A, Makimura K, de Hoog S, Shidfar MR, Zaini F, Eshraghian M, et al. Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey. Medical mycology. 2013;51(2):203-7.

Havlickova B, Czaika VA, Friedrich M. Epidemiological trends in skin mycoses worldwide. Mycoses. 2008;51(s4):2-15.

Adams BB. Tinea corporis gladiatorum. Journal of the American Academy of Dermatology. 2002;47(2):286-90.

Baldo A, Monod M, Mathy A, Cambier L, Bagut E, Defaweux V, et al. Mechanisms of skin adherence and invasion by dermatophytes. Mycoses. 2012;55(3):218-23.

Monod M, Lechenne B, Jousson O, Grand D, Zaugg C, Stöcklin R, et al. Aminopeptidases and dipeptidyl-peptidases secreted by the dermatophyte Trichophyton rubrum. Microbiology. 2005;151(1):145-55.

Garg A, Müller J. Fungitoxicity of fatty acids against dermatophytes. Mycoses. 1993;36(1‐2):51-63.

Hashemi SJ, Sarasgani MR, Zomorodian K. A comparative survey of serum androgenic hormones levels between male patients with dermatophytosis and normal subjects. Japanese journal of infectious diseases. 2004;57(2):60-2.

Kunert J. Inorganic sulphur sources for the growth of the dermatophyte Microsporum gypseum. Folia microbiologica. 1981;26(3):196-200.

Martinez-Rossi NM, Peres NT, Rossi A. Antifungal resistance mechanisms in dermatophytes. Mycopathologia. 2008;166(5-6):369.

Kahlos K, Tikka V. Antifungal activity of cysteine, its effect on C-21 oxygenated lanosterol derivatives and other lipids in Inonofus obliquus, in vitro. Applied Microbiology and Biotechnology. 1994;42(2):385-90.

Kelly BP. Superficial fungal infections. Pediatrics in review/American Academy of Pediatrics. 2012;33(4):e22-37.

Beller M, Gessner BD. An outbreak of tinea corporis gladiatorum on a high school wrestling team. Journal of the American Academy of Dermatology. 1994;31(2):197-201.

Fari E, Gräser Y. An epidemic of tinea corporis caused by Trichophyton tonsurans among children (wrestlers) in Germany. Mycoses. 2000;43(5‐6):191-6.

Philpot CM. The use of nutritional tests for the differentiation of dermatophytes. Sabouraudia. 1977;15(2):141-50.

Giddey K, Monod M, Barblan J, Potts A, Waridel P, Zaugg C, et al. Comprehensive analysis of proteins secreted by Trichophyton rubrum and Trichophyton violaceum under in vitro conditions. Journal of proteome research. 2007;6(8):3081-92.

Monod M, Capoccia S, Léchenne B, Zaugg C, Holdom M, Jousson O. Secreted proteases from pathogenic fungi. International Journal of Medical Microbiology. 2002;292(5-6):405-19.

Gan EY, Cai SC, Tang MB. Filaggrin and Skin Infections. Filaggrin: Springer; 2014. p. 279-91.

Klein DT. Morphological response of Trichophyton mentagrophytes to methionine. Microbiology. 1963;31(1):91-6.

Sarasgani M, Firoozrai M, Hashemi SJ. The effect of amino acids on the growth of Microsporum canis and Trichophyton schoenleinii. Tehran University Medical Journal TUMS Publications. 2008;66(3):158-64.

Gharachorlou A, Hashemi S, Gharachorlou S, Ghalekandi JG, Asghari A. Survey of tryptophan effects on trichophyton verrucosum growth in normal and dermatophytic patients under in vivo and in vitro conditions. Advances in Environmental Biology. 2011:1984-8.

Pandey D, Chandra H, Tripathi N, Dixit S. Antimycotic activity of some amino acids against dermatophytes. Arzneimittel-Forschung. 1984;34(5):554-6.

Norris HA, Elewski BE, Ghannoum MA. Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method. Journal of the American Academy of Dermatology. 1999;40(6):S9-S13.

Jessup C, Warner J, Isham N, Hasan I, Ghannoum M. Antifungal susceptibility testing of dermatophytes: establishing a medium for inducing conidial growth and evaluation of susceptibility of clinical isolates. Journal of clinical microbiology. 2000;38(1):341-4.

Stockdale PM. Nutritional requirements of the dermatophytes. Biological reviews. 1953;28(1):84-104.

Minocha Y, Pasricha J, Mohapatra L, Kandhari K. Proteolytic activity of dermatophytes and its role in the pathogenesis of skin lesions. Sabouraudia: Journal of Medical and Veterinary Mycology. 1972;10(1):79-85.

Bakhshi H HS, Mahmoodi M, Mansoori P, Zareei M. Study of free amino acids in stratum corneum and plasma in patients with dermatophytosis and normal subjects. International Journal of Advanced Research. 2015;3(1):643-53.

Takahashi M, Tezuka T. The content of free amino acids in the stratum corneum is increased in senile xerosis. Archives of dermatological research. 2004;295(10):448-52.

Katagiri C, Sato J, Nomura J, Denda M. Changes in environmental humidity affect the water-holding property of the stratum corneum and its free amino acid content, and the expression of filaggrin in the epidermis of hairless mice. Journal of dermatological science. 2003;31(1):29-35.


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